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Analyte Information
IFN-γ
Interferon-γ(IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γby helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γis typically associated with effective host defense against intracellular pathogens.
ELISABASIC kit for quantification of IFN-γin solution
The human IFN-γELISABASIC kit is ideal for users who want the flexibility of setting up their own ELISA assay. This product enables quantification of human IFN-γin solution, e.g. in cell culture supernatant. The kit contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-HRP, ELISA standard and standard reconstitution buffer. Plates and substrate are not included.
Human IFN-γELISABASIC kits are also available with Streptavidin-ALP.
Serum/Plasma samples
Analysis of serum/plasma samples with this kit requires the use of ELISA diluent for dilution of the samples, standard and detection antibody. The diluent prevents false positive read-outs which may be caused by interference of heterophilic antibodies found in serum and plasma. The ELISA diluent is available from Mabtech. See Related products.
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艾美捷人搅扰素Y ELISA开发试剂盒,用于定量溶液中的IFN-γ
人搅扰素Y ELISA开发试剂盒十分合适期望灵敏设置自己的ELISA检测的用户。该产品可以对溶液中的人IFN-γ 进行定量,例如在细胞培养上清液中。该试剂盒包括一对匹配的单克隆捕获和检测抗体、Streptavidin-HRP、ELISA规范和规范重组缓冲液。不包括板和基板。人搅扰素Y ELISA开发试剂盒也可与Streptavidin-ALP一同运用。
血清/血浆样本
运用艾美捷人搅扰素Y ELISA开发试剂盒剖析血清/血浆样品,需求运用ELISA稀释剂来稀释样品、规范品和检测抗体。稀释剂可防止因为血清和血浆中发现的异嗜性抗体的搅扰而导致的假阳性读数。ELISA稀释剂可从Mabtech取得。仅供研讨运用(RUO)。不用于确诊进程。
艾美捷人搅扰素Y ELISA开发试剂盒规范曲线的制作:
艾美捷人搅扰素Y ELISA开发试剂盒内容:
捕获mAb (1-D1K)
生物素化检测mAb (7-B6-1)
链霉亲和素-HRP
重组人IFN-γELISA规范品
规范重组缓冲液
使用:酶联免疫吸附实验
反响性:人类
规范规模:4-400皮克/毫升
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