Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. APC-Cy7 is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at~780 nm.

AAT Bioquest offers this preactivated APC-Cy7 to facilitate the APC-Cy7 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-Cy7 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-Cy7 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.

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别藻蓝蛋白(APC)是一种从蓝绿藻螺旋藻中分离出来的藻胆蛋白。与其他藻胆蛋白相同，APC是荧光的，具有J高的吸收率和高量子功率。它是一种能够经过惯例蛋白质交联技能轻松与抗体和其他蛋白质衔接而不改动其光谱特性的蛋白质。APC-Cy7是流式细胞术中常用的色彩。其主要吸收峰在651 nm，发射峰在~780 nm。

艾美捷预激活APC-Cy7串联以促进APC-Cy7与抗体和其他蛋白质（如链霉亲和素和其他二级试剂）的串联偶联。该预激活APC-Cy7串联已准备好进行偶联，与传统繁琐的根据SMCC的偶联化学比较，其产值要高得多。

艾美捷预激活APC-Cy7串联一般储藏溶液制备：

将特定质量的ReadiUse™ 预活化APC-Cy7 Tandem复溶至给定浓度所需的恰当溶剂体积。请注意，体积仅用于制备储藏溶液。

艾美捷预激活APC-Cy7串联组件：

A：ReadiUse™ 预激活APC-Cy7串联1瓶（1毫克）

B：Buccutite™MTA1瓶(100 µg)

C：旋转脱盐柱不包括

光谱特性：

消光系数（cm -1 M -1）700000

激起（纳米）651

发射（纳米）779

贮存、安全和处理：

H-短语H303、H313、H333

风险符号XN

有或许的运用仅供研讨运用(RUO)

R-短语R20、R21、R22

UNSPSC12171501