几种常见的细胞污染类型及处理办法

版块：机械制造类型：普通作者：化工产业技术分享查看：87 回复：0 获赞：0 时间：2022-05-18 19:05:41

凡混入细胞培养环境中对细胞生计有害的成分和构成细胞不纯的异物都应视为细胞污染，细胞污染不能彻底被消除，但能削减其产生的频率和结果的严重性。

本文将扼要介绍几种常见的细胞污染类型及处理办法。

常见细胞污染类型

细胞细菌污染

特征：巨细在0.5~5 μm，比动物细胞小许多。多呈球状或杆状，形状规矩，散布均匀；增殖速度快，一般细菌污染后48~72小时爆发式增殖；养分耗费快，培养基很快变黄，变污浊；镜下调查有显着的定向运动。

处理办法：轻度细菌污染可用10X双抗清洗处理，重度细菌污染主张消杀后丢掉。

细胞真菌污染

特征：细胞真菌污染呈链球状或丝状。常构成肉眼可见的菌落，培养基色彩无变化或变紫，仅对部分细胞影响较大，其他地方成长正常。会构成孢子，简单污染其他细胞。

处理办法：细胞真菌污染较难*，不主张保存，主张消杀后丢掉。

细胞支原体污染

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<section style="white-space: normal; background-color: rgb(255, 255, 255); font-family: PingFangSC-light; letter-spacing: 1px; padding-right: 5px; padding-left: 5px; box-sizing: border-box;"><p style="font-family: inherit; padding-bottom: 10px; padding-top: 10px; margin-top: 0px; margin-bottom: 0px; line-height: 1.8; border: none; box-sizing: border-box;"><span id="GL0" style="outline: none; font-family: inherit;">凡混入细胞培养环境中对细胞生计有害的成分和构成细胞不纯的异物都应视为细胞污染，细胞污染不能彻底被消除，但能削减其产生的频率和结果的严重性。</span></p></section><p><br/></p><section style="white-space: normal; font-family: 微软雅黑; background-color: rgb(255, 255, 255); font-size: 12px; box-sizing: border-box;"></section><section style="white-space: normal; background-color: rgb(255, 255, 255); font-family: PingFangSC-light; letter-spacing: 1px; padding-right: 5px; padding-left: 5px; box-sizing: border-box;"><p style="font-family: inherit; padding-bottom: 10px; padding-top: 10px; margin-top: 0px; margin-bottom: 0px; line-height: 1.8; border: none; box-sizing: border-box;"><strong>本文将扼要介绍几种常见的细胞污染类型及处理办法。</strong></p></section><section style="font-size: 16px; white-space: normal; font-family: 微软雅黑; background-color: rgb(255, 255, 255); box-sizing: border-box;"></section><section style="font-size: 16px; white-space: normal; font-family: 微软雅黑; background-color: rgb(255, 255, 255); text-align: center; justify-content: center; margin: 30px 0px 10px; box-sizing: border-box;"><section style="display: inline-block; width: auto; vertical-align: top; background-color: rgba(32, 100, 87, 0.1); border-width: 0px; min-width: 10%; max-width: 100%; height: auto; padding-right: 10px; padding-bottom: 5px; padding-left: 10px; box-sizing: border-box;"><section style="margin-top: 0px; margin-right: 0px; margin-left: 0px; box-sizing: border-box;"><section style="display: inline-block; width: 187.983px; vertical-align: top; border-style: solid; border-width: 1px; border-color: rgb(32, 100, 87); box-sizing: border-box;"><section style="margin-top: 0px; font-size: 0px; margin-right: 0px; margin-left: 0px; box-sizing: border-box;"><section style="display: inline-block; width: 185.994px; vertical-align: top; border-top: 1px solid rgb(32, 100, 87); border-top-left-radius: 0px; box-sizing: border-box;"><section style="margin-top: 0px; text-align: left; margin-right: 0px; margin-left: 0px; justify-content: flex-start; transform: translate3d(-15px, 0px, 0px); box-sizing: border-box;"></section></section></section><section style="margin-top: 4px; margin-right: 0px; margin-left: 0px; box-sizing: border-box;"><section style="color: rgb(32, 100, 87); padding-right: 15px; padding-left: 15px; font-family: PingFangSC-ultralight; letter-spacing: 2px; box-sizing: border-box;"><p style="font-family: inherit; padding-bottom: 10px; padding-top: 10px; margin-top: 0px; margin-bottom: 0px; line-height: 1.8; border: none; box-sizing: border-box;"><strong>常见细胞污染类型</strong></p></section></section></section></section></section></section><section style="white-space: normal; font-family: 微软雅黑; background-color: rgb(255, 255, 255); font-size: 12px; box-sizing: border-box;"></section><section style="font-size: 16px; white-space: normal; font-family: 微软雅黑; background-color: rgb(255, 255, 255); margin: 10px 0px -8px; box-sizing: border-box;"><section style="display: inline-block; width: auto; vertical-align: top; border-width: 0px; min-width: 10%; max-width: 100%; height: auto; box-sizing: border-box;"><section style="box-sizing: border-box;"><section style="display: flex; flex-flow: row nowrap; margin-right: 0px; margin-left: 0px; justify-content: flex-start; box-sizing: border-box;"><section style="display: inline-block; vertical-align: middle; width: auto; flex: 0 0 0%; align-self: center; height: auto; margin-right: 5px; margin-left: -11px; box-sizing: border-box;"><section style="transform: rotateZ(90deg); box-sizing: border-box;"><section style="display: flex; flex-flow: row nowrap; text-align: center; justify-content: center; margin-right: 0px; margin-left: 0px; box-sizing: border-box;"><section style="display: inline-block; width: 28px; vertical-align: top; background-color: rgb(30, 125, 106); flex: 0 0 auto; height: auto; line-height: 0; align-self: flex-start; box-sizing: border-box;"><section style="margin-right: 0px; margin-left: 0px; box-sizing: border-box;"><section style="display: inline-block; max-width: 100%; vertical-align: middle; line-height: 0; width: 27.9972px; height: auto; box-sizing: border-box;"></section></section></section></section></section></section><section style="display: inline-block; vertical-align: middle; width: auto; align-self: center; min-width: 10%; max-width: 100%; flex: 0 0 auto; height: auto; background-color: rgb(245, 245, 245); margin-right: 7px; margin-left: -11px; box-sizing: border-box;"><section style="margin-right: 0px; margin-left: 0px; box-sizing: border-box;"><section style="color: rgb(32, 100, 87); font-size: 15px; padding-right: 15px; padding-left: 15px; font-family: PingFangSC-light; letter-spacing: 2px; box-sizing: border-box;"><p style="font-family: inherit; padding-bottom: 10px; padding-top: 10px; margin-top: 0px; margin-bottom: 0px; line-height: 1.8; border: none; box-sizing: border-box;"><strong>细胞细菌污染</strong></p></section></section></section></section></section></section></section><section style="white-space: normal; font-family: 微软雅黑; background-color: rgb(255, 255, 255); font-size: 12px; box-sizing: border-box;"></section><section style="font-size: 16px; white-space: normal; font-family: 微软雅黑; background-color: rgb(255, 255, 255); box-sizing: border-box;"><section style="display: flex; flex-flow: row nowrap; box-sizing: border-box;"><section style="display: inline-block; width: auto; vertical-align: top; border-left: 1px dashed rgb(59, 149, 115); border-bottom-left-radius: 0px; flex: 100 100 0%; align-self: flex-start; height: auto; margin-left: 10px; box-sizing: border-box;"><section style="margin-right: 0px; margin-bottom: 11px; margin-left: 0px; box-sizing: border-box;"><section style="padding-right: 8px; padding-left: 8px; font-size: 14px; font-family: PingFangSC-light; letter-spacing: 1px; box-sizing: border-box;"><p style="font-family: inherit; padding-bottom: 10px; padding-top: 10px; margin-top: 0px; margin-bottom: 0px; line-height: 1.8; border: none; box-sizing: border-box;"><span id="GL4" style="outline: none; font-family: inherit;"><span style="outline: none; font-family: inherit; color: rgb(32, 100, 87); box-sizing: border-box;"><strong>特征：</strong></span>巨细在0.5~5 μm，比动物细胞小许多。多呈球状或杆状，形状规矩，散布均匀；增殖速度快，一般细菌污染后48~72小时爆发式增殖；养分耗费快，培养基很快变黄，变污浊；镜下调查有显着的定向运动。</span></p></section></section></section></section></section><section style="white-space: normal; font-family: 微软雅黑; background-color: rgb(255, 255, 255); font-size: 12px; box-sizing: border-box;"></section><section style="font-size: 16px; white-space: normal; font-family: 微软雅黑; background-color: rgb(255, 255, 255); margin-top: 10px; margin-bottom: 10px; box-sizing: border-box;"><section style="display: inline-block; width: 917.997px; border-width: 2px; border-style: dashed; border-color: rgb(192, 200, 209); padding: 10px; border-radius: 0.7em; box-sizing: border-box;"><section style="font-size: 14px; font-family: PingFangSC-light; letter-spacing: 1px; padding-right: 5px; padding-left: 5px; box-sizing: border-box;"><p style="font-family: inherit; padding-bottom: 10px; padding-top: 10px; margin-top: 0px; margin-bottom: 0px; line-height: 1.8; border: none; box-sizing: border-box;"><span style="outline: none; font-family: inherit; color: rgb(32, 100, 87); box-sizing: border-box;"><strong>处理办法</strong></span><strong>：</strong><span id="GL7" style="outline: none; font-family: inherit; box-sizing: border-box;">轻度细菌污染可用<span style="outline: none; font-family: inherit; box-sizing: border-box;"><strong>10X双抗</strong></span>清洗处理，重度细菌污染主张消杀后丢掉。</span></p></section></section></section><section style="font-size: 16px; white-space: normal; font-family: 微软雅黑; background-color: rgb(255, 255, 255); height: auto; box-sizing: border-box;"><section style="display: flex; flex-flow: row nowrap; margin-right: 0px; margin-bottom: 20px; margin-left: 0px; box-sizing: border-box;"><section style="display: inline-block; 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letter-spacing: 2px; box-sizing: border-box;"><p style="font-family: inherit; padding-bottom: 10px; padding-top: 10px; margin-top: 0px; margin-bottom: 0px; line-height: 1.8; border: none; box-sizing: border-box;"><strong>细胞真菌污染</strong></p></section></section></section></section></section></section></section><section style="font-size: 16px; white-space: normal; font-family: 微软雅黑; background-color: rgb(255, 255, 255); box-sizing: border-box;"></section><section style="font-size: 16px; white-space: normal; font-family: 微软雅黑; background-color: rgb(255, 255, 255); box-sizing: border-box;"><section style="display: flex; flex-flow: row nowrap; box-sizing: border-box;"><section style="display: inline-block; width: auto; vertical-align: top; border-left: 1px dashed rgb(59, 149, 115); border-bottom-left-radius: 0px; flex: 100 100 0%; align-self: flex-start; height: auto; margin-left: 10px; box-sizing: border-box;"><section style="margin-right: 0px; margin-bottom: 11px; margin-left: 0px; box-sizing: border-box;"><section style="padding-right: 8px; padding-left: 8px; font-size: 14px; font-family: PingFangSC-light; letter-spacing: 1px; box-sizing: border-box;"><p style="font-family: inherit; padding-bottom: 10px; padding-top: 10px; margin-top: 0px; margin-bottom: 0px; line-height: 1.8; border: none; box-sizing: border-box;"><span id="GL9" style="outline: none; font-family: inherit;"><span style="outline: none; font-family: inherit; color: rgb(32, 100, 87); box-sizing: border-box;"><strong>特征</strong></span><strong>：</strong>细胞真菌污染呈链球状或丝状。常构成肉眼可见的菌落，培养基色彩无变化或变紫，仅对部分细胞影响较大，其他地方成长正常。会构成孢子，简单污染其他细胞。</span></p></section></section></section></section></section><section style="white-space: normal; font-family: 微软雅黑; background-color: rgb(255, 255, 255); font-size: 12px; box-sizing: border-box;"></section><section style="font-size: 16px; white-space: normal; font-family: 微软雅黑; background-color: rgb(255, 255, 255); margin-top: 10px; margin-bottom: 10px; box-sizing: border-box;"><section style="display: inline-block; width: 917.997px; border-width: 2px; border-style: dashed; border-color: rgb(192, 200, 209); padding: 10px; border-radius: 0.7em; box-sizing: border-box;"><section style="font-size: 14px; font-family: PingFangSC-light; letter-spacing: 1px; padding-right: 5px; padding-left: 5px; box-sizing: border-box;"><p style="font-family: inherit; padding-bottom: 10px; padding-top: 10px; margin-top: 0px; margin-bottom: 0px; line-height: 1.8; border: none; box-sizing: border-box;"><span id="GL10" style="outline: none; font-family: inherit; color: rgb(32, 100, 87); box-sizing: border-box;"><strong>处理办法</strong>：</span><span id="GL11" style="outline: none; font-family: inherit; box-sizing: border-box;">细胞真菌污染较难*，不主张保存，主张消杀后丢掉。</span></p></section></section></section><section style="font-size: 16px; white-space: normal; font-family: 微软雅黑; background-color: rgb(255, 255, 255); height: auto; box-sizing: border-box;"><section style="display: flex; flex-flow: row nowrap; margin-right: 0px; margin-bottom: 20px; margin-left: 0px; box-sizing: border-box;"><section style="display: inline-block; 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