细胞污染的区分

版块：机械制造类型：普通作者：化工产业技术分享查看：92 回复：0 获赞：0 时间：2022-05-18 18:58:06

细胞污染的区分

图片4.png

细胞污染有很多种不同的类型，不同状况的细胞污染其体现也不同，检测办法也有所不同。不过大部分的细胞污染，用肉眼加镜检就可以处理啦！

细胞污染一般分细菌污染，真菌污染，支原体污染，黑胶虫等。

1.细菌污染

pH升高，培育基变黄，培育基严峻污浊，镜下可见细菌游动。

2.真菌污染

培育液短期内多不污浊，有肉眼可见漂浮物，镜下细胞间有菌丝体，成长变慢，时间长细胞逝世。

3.支原体污染

支原体污染后，细胞成长一般无可见改变，在光学显微镜下不可见，极易被忽视，往往直到污染十分严峻时才干发现，检测需求经过特定的检测办法来进行。现在，支原体检测的办法有很多种，如支原体别离培育、DNA荧光染色、ELISA法（检测支原体特异酶表达）和PCR法（扩增支原体rRNA序列保存区）等。

4.黑胶虫污染

培育液不污浊，细胞成长影响不大，镜下有小黑点做布朗运动。

小结：培育细胞中的细菌污染、酵母污染或霉菌污染都在光学显微镜下可见，但支原体污染在光学显微镜下不可见，有必要经过特定的检测办法进行检测。

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padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></section><section data-brushtype="text" style="margin: 0px; padding: 12px 2em 0px; outline: 0px; max-width: 100%; box-sizing: border-box; font-size: 16px; letter-spacing: 1.5px; overflow-wrap: break-word !important;"><h1 style="margin: 0px; padding: 0px; outline: 0px; font-weight: 400; font-size: 16px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 28px; letter-spacing: 1px;"><strong style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-family: 宋体; box-sizing: border-box !important; overflow-wrap: break-word !important;"><strong style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;">细胞污染的区分</strong></span></strong></span><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 28px;"><strong style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 宋体;"></span></strong></span></h1><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; box-sizing: border-box !important; overflow-wrap: break-word !important;"><br data-filtered="filtered" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></p></section><section style="margin: 0px 17px 0px 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box; overflow-wrap: break-word !important; width: 1px; background: rgb(51, 51, 51); height: 40px; overflow: hidden; transform: rotate(30deg);"><br data-filtered="filtered" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></section></section></section><section style="margin: -1.4em 0px 0px -3em; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box; overflow-wrap: break-word !important; width: 3em; border-bottom: 1px solid rgb(51, 51, 51); height: 1px; overflow: hidden;"><br data-filtered="filtered" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></section><section style="margin: 0px -3em -1.4em 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box; overflow-wrap: break-word !important; float: right; width: 3em; border-bottom: 1px solid rgb(51, 51, 51); height: 1px; overflow: hidden;"><br data-filtered="filtered" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; color: rgb(51, 51, 51); font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; white-space: normal; background-color: rgb(255, 255, 255); box-sizing: border-box !important; overflow-wrap: break-word !important;"/></section></section></section></section></section><p><br/></p><p style="text-align: center"><img style="max-width: 800px; max-height: 300%; width: 322px; height: 181px;" src="https://img78.chem17.com/9/20220223/637812068334406600682.png" title="图片4.png" alt="图片4.png" width="322" height="181"/></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; background: rgb(255, 255, 255); line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-family: 微软雅黑; caret-color:red letter-spacing: 1px; box-sizing: border-box !important; overflow-wrap: break-word !important;">细胞污染有很多种不同的类型，不同状况的细胞污染其体现也不同，检测办法也有所不同。不过大部分的细胞污染，用肉眼加镜检就可以处理啦！</span></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; background: rgb(255, 255, 255); line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><br data-filtered="filtered" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; background: rgb(255, 255, 255); line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; color: rgb(49, 133, 155); letter-spacing: 1px;"><strong style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-family: 微软雅黑; box-sizing: border-box !important; overflow-wrap: break-word !important;">细胞污染一般分细菌污染，真菌污染，支原体污染，黑胶虫等。</span></strong></span></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; background: rgb(255, 255, 255); line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><br data-filtered="filtered" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; background: rgb(255, 255, 255); line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; color: rgb(49, 133, 155); font-size: 16px; letter-spacing: 1px;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-family: 微软雅黑; box-sizing: border-box !important; overflow-wrap: break-word !important;"><strong style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;">1.</strong><strong style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"></strong></span><strong style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-family: 微软雅黑; box-sizing: border-box !important; overflow-wrap: break-word !important;"><strong style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;">细菌污染</strong></span></strong></span></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; background: rgb(255, 255, 255); line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-family: 微软雅黑; letter-spacing: 1px; box-sizing: border-box !important; overflow-wrap: break-word !important;">pH升高，培育基变黄，培育基严峻污浊，镜下可见细菌游动。</span></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; background: rgb(255, 255, 255); line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><br data-filtered="filtered" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 微软雅黑; color: rgb(49, 133, 155); font-size: 16px; letter-spacing: 1px;"><strong style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;">2.真菌污染</strong></span></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; background: rgb(255, 255, 255); line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-family: 微软雅黑; letter-spacing: 1px; box-sizing: border-box !important; overflow-wrap: break-word !important;">培育液短期内多不污浊，有肉眼可见漂浮物，镜下细胞间有菌丝体，成长变慢，时间长细胞逝世。</span></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; background: rgb(255, 255, 255); line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><br data-filtered="filtered" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 微软雅黑; color: rgb(49, 133, 155); font-size: 16px; letter-spacing: 1px;"><strong style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;">3.支原体污染</strong></span></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; background: rgb(255, 255, 255); line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; letter-spacing: 1px;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-family: 微软雅黑; box-sizing: border-box !important; overflow-wrap: break-word !important;">支原体污染后，细胞成长一般无可见改变，在光学显微镜下不可见，极易被忽视，往往直到污染十分严峻时才干发现，检测需求经过特定的检测办法来进行。</span><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-family: 微软雅黑; box-sizing: border-box !important; overflow-wrap: break-word !important;">现在，支原体检测的办法有很多种，如支原体别离培育、DNA荧光染色、ELISA法（检测支原体特异酶表达）和PCR法（扩增支原体rRNA序列保存区）等。</span></span></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; background: rgb(255, 255, 255); line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><br data-filtered="filtered" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 微软雅黑; color: rgb(49, 133, 155); font-size: 16px; letter-spacing: 1px;"><strong style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;">4.黑胶虫污染</strong></span></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; background: rgb(255, 255, 255); line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-family: 微软雅黑; letter-spacing: 1px; box-sizing: border-box !important; overflow-wrap: break-word !important;">培育液不污浊，细胞成长影响不大，镜下有小黑点做布朗运动。</span></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; background: rgb(255, 255, 255); line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><br data-filtered="filtered" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; background: rgb(255, 255, 255); line-height: 1.75em; box-sizing: border-box !important; overflow-wrap: break-word !important;"><strong style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-family: 微软雅黑; color: rgb(49, 133, 155); text-decoration-line: underline; font-size: 15px; letter-spacing: 1px; box-sizing: border-box !important; overflow-wrap: break-word !important;">小结：</span></strong><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-family: 微软雅黑; color: rgb(49, 133, 155); text-decoration-line: underline; font-size: 15px; letter-spacing: 1px; box-sizing: border-box !important; overflow-wrap: break-word !important;">培育细胞中的细菌污染、酵母污染或霉菌污染都在光学显微镜下可见，但支原体污染在光学显微镜下不可见，有必要经过特定的检测办法进行检测。</span></p><p style="margin-top: 0px; margin-bottom: 0px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; vertical-align: inherit; background: rgb(255, 255, 255); box-sizing: border-box !important; overflow-wrap: break-word !important;"><br data-filtered="filtered" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; color: rgb(51, 51, 51); font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; white-space: normal; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></p><p style="margin-right: 5px; margin-left: 5px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgb(51, 51, 51); font-family: -apple-system, BlinkM

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