生物制品中快速精准检测支原体污染的办法

版块：机械制造类型：普通作者：化工产业技术分享查看：83 回复：0 获赞：0 时间：2022-05-18 18:50:49

生物制品中快速精准检测支原体污染的办法

在生物制品制备进程中需求严厉的质检方能完成产品的安全可靠，如基因医治产品、细胞医治产品、生物疫苗等，这些生物制品大多以细胞为载体制备或构建，在出产制备进程易遭到支原体污染。

图片来历：摄图网

据文献报导，支原体污染在贮存细胞系中发生率约为 15%-35%，在生物制药职业中约为0.44%-6.70%。在这些污染事情中，95%的支原体品种是以下几种：口腔支原体、精氨酸支原体、猪鼻支原体、发酵支原体、人型支原体或莱氏无胆原体等^[^1]。

支原体污染关于生物制备企业来讲就好像梦魇一般，不只导致产品品质下降；还会下降表达水平并终究导致产值下降，如污染了精氨酸支原体的抗体出产所用CHO DG44反响器中，发现在污染2-6天后开端发生副作用，使得细胞成长速率下降并导致反响系统内DO和pH改动。一起支原体污染也会对患者发生不良的副作用。快速、准确的检测支原体的存在已然火烧眉毛，现在国内外药典引荐的支原体检测办法主要是根据培育法、NAT(核酸扩增)法、指示细胞培育法^[^2]。

^{表：药典检测法优缺陷比照[2,3]}

办法类型	长处	缺陷
培育法	高灵敏度，可检测到0.1CFU/mL	时刻过长，整个进程需28天不同支原体需不同培育基进行培育，某些支原体难以培育
NAT法（核酸扩增）	快速到达10CFU/mL以下的检测限	需求特定提取试剂盒和检测设备 DNA的提取质量和功率影响成果的可信任度需求其他办法如培育法进行可比验证
指示细胞培育法	TEC传感器 \| 猫掌外链免责声明 1、小猪外链里发表的文章仅代表作者本人的观点，与本网站立场无关。 2、小猪外链网资源分享仅为个人学习、交流之用，同时向原著作者表达敬意。 3、小猪外链网仅提供信息存储空间服务，小猪外链网信息均来源于用户自行发布，不承担任何法律责任。如发现本站有涉嫌抄袭侵权/违法违规的内容，可以按照《小猪外链网文章侵权处理流程》进行处理，同时向原著作者表达敬意。 4、内容由网友自主上传，如有侵权、违规请联系邮箱616859395@qq.com进行处理。如果觉得本文还不错请点个赞或者打赏点金币哦~ 拒绝伸手党，拿走请回复，尊重楼主，尊重你我他~ 回复列表默认热门正序倒序生物制品中快速精准检测支原体污染的办法<p><br/></p><section data-role="paragraph" data-mpa-powered-by="yiban.io" style="margin: 0px; padding: 0px; white-space: normal; outline: 0px; max-width: 100%; color: rgb(51, 51, 51); font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; background-color: rgb(255, 255, 255); overflow-wrap: break-word !important; box-sizing: border-box !important;"><section style="overflow-wrap: break-word !important; margin: 0px 8px; padding: 0px; outline: 0px; max-width: 100%; vertical-align: inherit; box-sizing: border-box !important;"><span style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; font-family: "times new roman", SimHei !important; outline: 0px; max-width: 100%; font-size: 15px; letter-spacing: 1px; box-sizing: border-box !important;">在生物制品制备进程中需求严厉的质检方能完成产品的安全可靠，如基因医治产品、细胞医治产品、生物疫苗等，这些生物制品大多以细胞为载体制备或构建，在出产制备进程易遭到支原体污染。</span><br style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important;"/></section></section><section style="margin: 0px 8px; padding: 0px; white-space: normal; outline: 0px; max-width: 100%; color: rgb(51, 51, 51); font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; text-align: justify; background-color: rgb(255, 255, 255); vertical-align: inherit; line-height: 1.75em; letter-spacing: 1px; overflow-wrap: break-word !important; box-sizing: border-box !important;"><span style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; font-family: "times new roman", SimHei !important; outline: 0px; max-width: 100%; font-size: 15px; box-sizing: border-box !important;"></span></section><section style="margin: 0px; padding: 0px; white-space: normal; outline: 0px; max-width: 100%; color: rgb(51, 51, 51); font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; background-color: rgb(255, 255, 255); overflow-wrap: break-word !important; box-sizing: border-box !important;"><section style="overflow-wrap: break-word !important; margin: 0px 8px; padding: 0px; outline: 0px; max-width: 100%; text-align: center; vertical-align: inherit; line-height: 0em; box-sizing: border-box !important;"><img src="uploadfiles/images/1429/20220518/185049_Rf20EP.jpg" title="1645174517148281.jpg" alt="1645174517148281.jpg" width="650" height="358" border="0" vspace="0" style="overflow-wrap: normal !important; margin: 0px; padding: 0px; max-width: 100%; border: 0px; outline-style: none; width: 650px; height: 358px;"/></section></section><section style="margin: 0px 8px; padding: 0px; white-space: normal; outline: 0px; max-width: 100%; color: rgb(51, 51, 51); font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; background-color: rgb(255, 255, 255); vertical-align: inherit; line-height: 1.75em; letter-spacing: 1px; text-align: center; overflow-wrap: break-word !important; box-sizing: border-box !important;"><span style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; font-family: "times new roman", SimHei !important; outline: 0px; max-width: 100%; font-size: 13px; box-sizing: border-box !important;">图片来历：摄图网</span><br style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important;"/></section><section style="margin: 0px 8px; padding: 0px; white-space: normal; outline: 0px; max-width: 100%; color: rgb(51, 51, 51); font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; text-align: justify; background-color: rgb(255, 255, 255); vertical-align: inherit; line-height: 1.75em; letter-spacing: 1px; overflow-wrap: break-word !important; box-sizing: border-box !important;"><br style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important;"/></section><section style="margin: 0px 8px; padding: 0px; white-space: normal; outline: 0px; max-width: 100%; color: rgb(51, 51, 51); font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; text-align: justify; background-color: rgb(255, 255, 255); vertical-align: inherit; line-height: 1.75em; letter-spacing: 1px; overflow-wrap: break-word !important; box-sizing: border-box !important;"><span style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; font-family: "times new roman", SimHei !important; outline: 0px; max-width: 100%; font-size: 15px; box-sizing: border-box !important;">据文献报导，支原体污染在贮存细胞系中发生率约为 15<span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; overflow-wrap: break-word !important; box-sizing: border-box !important;">%</span>-35%，在生物制药职业中约为0.44<span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; overflow-wrap: break-word !important; box-sizing: border-box !important;">%</span>-6.70%。在这些污染事情中，95%的支原体品种是以下几种：口腔支原体、精氨酸支原体、猪鼻支原体、发酵支原体、人型支原体或莱氏无胆原体等</span><span style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; font-family: "times new roman", SimHei !important; outline: 0px; max-width: 100%; font-size: 11px; box-sizing: border-box !important;"><sup style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important;"><span style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; font-family: "Times New Roman"; outline: 0px; max-width: 100%; vertical-align: super; box-sizing: border-box !important;">[</span></sup><sup style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; vertical-align: super; overflow-wrap: break-word !important; box-sizing: border-box !important;">1]</span></sup></span><span style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; font-family: "times new roman", SimHei !important; outline: 0px; max-width: 100%; font-size: 15px; box-sizing: border-box !important;">。</span></section><section style="margin: 0px 8px; padding: 0px; white-space: normal; outline: 0px; max-width: 100%; color: rgb(51, 51, 51); font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; text-align: justify; background-color: rgb(255, 255, 255); vertical-align: inherit; line-height: 1.75em; letter-spacing: 1px; overflow-wrap: break-word !important; box-sizing: border-box !important;"><br style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important;"/></section><section style="margin: 0px 8px; padding: 0px; white-space: normal; outline: 0px; max-width: 100%; color: rgb(51, 51, 51); font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; text-align: justify; background-color: rgb(255, 255, 255); vertical-align: inherit; line-height: 1.75em; letter-spacing: 1px; overflow-wrap: break-word !important; box-sizing: border-box !important;"><span style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; font-family: "times new roman", SimHei !important; outline: 0px; max-width: 100%; font-size: 15px; box-sizing: border-box !important;"></span><span style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; font-family: "times new roman", SimHei !important; outline: 0px; max-width: 100%; font-size: 15px; box-sizing: border-box !important;">支原体污染关于生物制备企业来讲就好像梦魇一般，不只导致产品品质下降；还会下降表达水平并终究导致产值下降，如污染了精氨酸支原体的抗体出产所用CHO DG44反响器中，发现在污染2-6天后开端发生副作用，使得细胞成长速率下降并导致反响系统内DO和pH改动。一起支原体污染也会对患者发生不良的副作用。快速、准确的检测支原体的存在已然火烧眉毛，现在国内外药典引荐的支原体检测办法主要是根据培育法、NAT(核酸扩增)法、<span style="margin: 0px; padding: 0px; font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; overflow-wrap: normal !important;">指示</span>细胞培育法<sup style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important;"><span style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; font-family: "Times New Roman"; outline: 0px; max-width: 100%; vertical-align: super; box-sizing: border-box !important;">[</span></sup><sup style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; vertical-align: super; overflow-wrap: break-word !important; box-sizing: border-box !important;">2]</span></sup>。</span><span style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; font-family: "times new roman", SimHei !important; outline: 0px; max-width: 100%; font-size: 15px; box-sizing: border-box !important;"></span></section><section style="margin: 0px 8px; padding: 0px; white-space: normal; outline: 0px; max-width: 100%; color: rgb(51, 51, 51); font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; text-align: center; background-color: rgb(255, 255, 255); vertical-align: inherit; line-height: 1.75em; letter-spacing: 1px; overflow-wrap: break-word !important; box-sizing: border-box !important;"><strong style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important;"><span style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; font-family: "times new roman", SimHei !important; outline: 0px; max-width: 100%; font-size: 15px; box-sizing: border-box !important;"><sup style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; vertical-align: super; overflow-wrap: break-word !important; box-sizing: border-box !important;"><p style="margin-top: 10px; margin-bottom: 10px; padding: 0px; overflow-wrap: normal !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; overflow-wrap: break-word !important; box-sizing: border-box !important;">表：药典检测法优缺陷比照[2,3]</span></p></span></sup></span></strong></section><section style="margin: 0px 8px; padding: 0px; white-space: normal; outline: 0px; max-width: 100%; color: rgb(51, 51, 51); font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; text-align: justify; background-color: rgb(255, 255, 255); vertical-align: inherit; line-height: 1.75em; letter-spacing: 1px; overflow-wrap: break-word !important; box-sizing: border-box !important;"><strong style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important;"><span style="overflow-wrap: break-word !important; margin: 0px; padding: 0px; font-family: "times new roman", SimHei !important; outline: 0px; max-width: 100%; font-size: 15px; box-sizing: border-box !important;"></span></strong><table cellspacing="0" align="left" width="894" style="max-width: 748px;"><tbody style="overflow-wrap: normal !important; margin: 0px; padding: 0px;"><tr class="firstRow" style="overflow-wrap: normal !important; margin: 0px; padding: 0px; height: 24px;"><td valign="top" style="margin: 0px; padding-right: 7px; padding-left: 7px; border: 1px solid rgb(221, 221, 221); background: rgb(227, 108, 9); overflow-wrap: normal !important;"><p style="overflow-wrap: normal !important;"><strong style="overflow-wrap: normal !important; margin: 0px; padding: 0px;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; color: rgb(255, 255, 255); overflow-wrap: break-word !important; box-sizing: border-box !important;">办法类型</span></strong></p></td><td valign="top" style="margin: 0px; padding-right: 7px; padding-left: 7px; border: 1px solid rgb(221, 221, 221); background: rgb(227, 108, 9); overflow-wrap: normal !important;"><p style="overflow-wrap: normal !important;"><strong style="overflow-wrap: normal !important; margin: 0px; padding: 0px;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; color: rgb(255, 255, 255); overflow-wrap: break-word !important; box-sizing: border-box !important;">长处</span></strong></p></td><td valign="top" style="margin: 0px; padding-right: 7px; padding-left: 7px; border: 1px solid rgb(221, 221, 221); background: rgb(227, 108, 9); word-break: break-all; overflow-wrap: normal !important;"><p style="overflow-wrap: normal !important;"><span style="margin: 0px; padding: 0px; color: rgb(255, 255, 255); overflow-wrap: normal !important;"><strong style="overflow-wrap: normal !important; margin: 0px; padding: 0px;"><span style="margin: 0px; padding: 0px; font-family: 宋体; font-size: 14px; overflow-wrap: normal !important;">缺陷</span></strong></span></p></td></tr><tr style="overflow-wrap: normal !important; margin: 0px; padding: 0px; height: 46px;"><td valign="top" style="margin: 0px; padding-right: 7px; padding-left: 7px; border-top: none rgb(221, 221, 221); border-right: 1px solid rgb(221, 221, 221); border-bottom: 1px solid rgb(221, 221, 221); border-left: 1px solid rgb(221, 221, 221); border-image: initial; overflow-wrap: normal !important;"><p style="overflow-wrap: normal !important;"><strong style="overflow-wrap: normal !important; margin: 0px; padding: 0px;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; overflow-wrap: break-word !important; box-sizing: border-box !important;">培育法</span></strong></p></td><td valign="top" style="margin: 0px; padding-right: 7px; padding-left: 7px; border-top: none rgb(221, 221, 221); border-right: 1px solid rgb(221, 221, 221); border-bottom: 1px solid rgb(221, 221, 221); border-left: 1px solid rgb(221, 221, 221); border-image: initial; overflow-wrap: normal !important;"><p style="overflow-wrap: normal !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; overflow-wrap: break-word !important; box-sizing: border-box !important;">高灵敏度</span><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; overflow-wrap: break-word !important; box-sizing: border-box !important;">，可检测到0.1CFU/mL</span></p></td><td valign="top" style="margin: 0px; padding-right: 7px; padding-left: 7px; border-top: none rgb(221, 221, 221); border-right: 1px solid rgb(221, 221, 221); border-bottom: 1px solid rgb(221, 221, 221); border-left: 1px solid rgb(221, 221, 221); border-image: initial; overflow-wrap: normal !important;"><p style="overflow-wrap: normal !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; overflow-wrap: break-word !important; box-sizing: border-box !important;">时刻过长，整个进程需28天</span></p><p style="overflow-wrap: normal !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; overflow-wrap: break-word !important; box-sizing: border-box !important;">不同支原体需不同培育基进行培育，某些支原体难以培育</span></p></td></tr><tr style="overflow-wrap: normal !important; margin: 0px; padding: 0px; height: 62px;"><td valign="top" style="margin: 0px; padding-right: 7px; padding-left: 7px; border-top: none rgb(221, 221, 221); border-right: 1px solid rgb(221, 221, 221); border-bottom: 1px solid rgb(221, 221, 221); border-left: 1px solid rgb(221, 221, 221); border-image: initial; overflow-wrap: normal !important;"><p style="overflow-wrap: normal !important;"><strong style="overflow-wrap: normal !important; margin: 0px; padding: 0px;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; overflow-wrap: break-word !important; box-sizing: border-box !important;">NAT法（核酸扩增）</span></strong></p></td><td valign="top" style="margin: 0px; padding-right: 7px; padding-left: 7px; border-top: none rgb(221, 221, 221); border-right: 1px solid rgb(221, 221, 221); border-bottom: 1px solid rgb(221, 221, 221); border-left: 1px solid rgb(221, 221, 221); border-image: initial; overflow-wrap: normal !important;"><p style="overflow-wrap: normal !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; overflow-wrap: break-word !important; box-sizing: border-box !important;">快速</span></p><p style="overflow-wrap: normal !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; overflow-wrap: break-word !important; box-sizing: border-box !important;">到达10CFU/mL以下</span><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; overflow-wrap: break-word !important; box-sizing: border-box !important;">的检测限</span></p></td><td valign="top" style="margin: 0px; padding-right: 7px; padding-left: 7px; border-top: none rgb(221, 221, 221); border-right: 1px solid rgb(221, 221, 221); border-bottom: 1px solid rgb(221, 221, 221); border-left: 1px solid rgb(221, 221, 221); border-image: initial; overflow-wrap: normal !important;"><p style="overflow-wrap: normal !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; overflow-wrap: break-word !important; box-sizing: border-box !important;">需求特定提取试剂盒和检测设备</span></p><p style="overflow-wrap: normal !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; overflow-wrap: break-word !important; box-sizing: border-box !important;">DNA的提取质量和功率影响成果的可信任度</span></p><p style="overflow-wrap: normal !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; overflow-wrap: break-word !important; box-sizing: border-box !important;">需求其他办法如培育法进行可比验证</span></p></td></tr><tr style="overflow-wrap: normal !important; margin: 0px; padding: 0px; height: 47px;"><td valign="top" style="margin: 0px; padding-right: 7px; padding-left: 7px; border-top: none rgb(221, 221, 221); border-right: 1px solid rgb(221, 221, 221); border-bottom: 1px solid rgb(221, 221, 221); border-left: 1px solid rgb(221, 221, 221); border-image: initial; overflow-wrap: normal !important;"><p style="overflow-wrap: normal !important;"><strong style="overflow-wrap: normal !important; margin: 0px; padding: 0px;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; overflow-wrap: break-word !important; box-sizing: border-box !important;">指示细胞培育法</span></strong></p></td><td valign="top" style="margin: 0px; padding-right: 7px; padding-left: 7px; border-top: none rgb(221, 221, 221); border-right: 1px solid rgb(221, 221, 221); border-bottom: 1px solid rgb(221, 221, 221); border-left: 1px solid rgb(221, 221, 221); border-image: initial; overflow-wrap: normal !important;"><p style="overflow-wrap: normal !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: 15px; ove 首 1 尾回复：生物制品中快速精准检测支原体污染的办法登录注册 or 暂无用户组退出等级：0级小猪币：游客：消息退出个人中心推广中心打赏站长在线充值后台控制面板推荐文章打造品牌差异化宏山激光奏响“服务三部曲” 洗发水哪个牌子好?kono丝柔滋养洗发水怎么样？ PPT不用太复杂——简约PPT模板推荐欺骗消费者如何赔偿自动气象站监测系统_智能农业气象站钣金加工时需要注意哪些问题？药店售卖假药怎么处罚和胖纸交朋友你也会慢慢变成一个胖纸，体适能健身培训教你减肥什么是成考？什么是自考？成考难吗？合同可以是口头的吗网站公告近期本站被人为恶意注册及发布垃圾帖，每一个发帖都会经过审核，一经发现违法或垃圾帖的用户，帖子将被删除或封号，请大家共同维护互联网环境，共创美好互联网未来。详细的发帖规则请阅读：《小猪外链网发帖规则》《小猪外链网最新金币规则》注:本站严禁发布灰色违禁违法内容,如发现立刻永久封号,如开通会员的概不退款。 Powered by 小猪外链网 8.3.15 ©2015 - 2025 小猪SEO外链平台备案号:浙ICP备17015142号免责申明：本网站内容由平台入驻会员撰写，除创始人账号外，其他观点仅代表作者本人，不代表小猪外链网立场。如果内容涉及侵犯其他公司、团体的利益、请联系小猪SEO外链网客服举证删除您的IP:10.1.69.136,2025-07-13 17:55:00,Processed in 0.31397 second(s). 各位站长请准守小猪SEO外链网的发帖规则，文明理性发言用户名：粉丝数：签名：资料关注好友消息塑料水分测定仪运用原理及枯燥失重常用的办法事例集 \| 保“洁”赋能——如春风电陆上换流站暖通体系使用赞(0) 赏分享分享取消赏已有0次打赏

外链

首页

网站外链

SEO优化

自动发外链

外链任务

转盘游戏

链接申请

外链版块

生物制品中快速精准检测支原体污染的办法