【技能共享】细胞活性检测办法大全

版块：机械制造类型：普通作者：化工产业技术分享查看：68 回复：0 获赞：0 时间：2022-05-18 18:04:58

细胞的活性判定在生物学和医学上具有很重要的含义。它是体外细胞培养技能中常用的一个检测目标。不同的细胞活性判定办法有各自不同详细的反响机理，但不管选用哪种办法，都使用了死细胞和活细胞在生理机能和性质上的差异。常用的办法有直接染色法，克隆构成实验，比色法等。

下面咱们就简略的了解一下常用的细胞活性检测办法的原理，特色，以及各自的优缺陷。

一、染色计数法

化学染色法
染色计数法是细胞培养中查看细胞死活zui常用的办法,直接使用死细胞和活细胞对染料的不同亲和力,查看细胞活性,能在光学显微镜下观察到染色成果.可分为两类:死细胞上色法和活细胞上色法.使死细胞上色的常用染料有台盼蓝,苯胺黑,伊红Y.能使活细胞上色的常用染料有结晶紫,亚甲基蓝,甲苯胺蓝等.其间zui常用的是台盼蓝染色法….细胞损害或逝世时,台盼蓝可穿透变性的细胞膜,与崩溃的DNA结合,使其上色,而活细胞能阻挠染料进入细胞内,故能够辨别死细胞与活细胞.经过细胞计数可得出细胞的存活率.
长处：便利有用，价格低廉，操作简略
缺陷：时刻不宜过长,不然部分活细胞也会上色,会搅扰计数.并且,细胞没有经过固定,形状不明晰.
荧光染色法
一些荧光染料对死细胞和活细胞也有不同的作用作用,使用荧光显微镜检测细胞活性.如碘化丙锭。(PI),被活细胞排挤但能穿透正在逝世或现已逝世细胞的细胞膜,因此活细胞不被染料染上色,只要死细胞或是凋亡细胞才干染上红.色.叶啶橙(AO)能透过质膜完好的细胞,嵌入细胞核DNA,使之宣布亮堂的绿色荧光.溴化乙锭(EB)仅能透过胞膜受损的细胞,嵌入核DNA,发橘红色荧光.也可使用AO—EB双染法判定细胞死活.
长处：这种检测办法比较传统的染料,具有灵敏度高,操作简洁,成果简单分辩等特色,并且使用双染法还能够分辩活细胞,凋亡前期细胞,凋亡晚期细胞,逝世细胞，在细胞凋亡的检测上有很广泛的使用。
缺陷：要求特别的仪器进行检测,如荧光显微镜,激光扫描共聚集显微镜或流式细胞仪.并且一般荧光染料具有毒性,操作作时需要带手套.

二、克隆构成实验

克隆构成实验是测定单个细胞增值才能的有用办法之一,其基本原理是单个细胞在体外继续割裂增殖6 次以上,其子孙所组成的细胞集体,称为克隆或集落.一般状况,每个克降可含50个以上的细胞,巨细在0.3~~I.0mm之间.经过计数克隆构成率,可对单个细胞的增值潜力做定量分析.常见的办法有平板克隆构成实验,软琼脂构成实验等.这种办法常见于抗肿瘤药物敏感性实验,肿瘤放射生物学实验等.

长处：在低剂量辐射下,克隆构成实验比 MTT和SRB法的敏感性更强.

缺陷：较为繁琐并耗l时,不适合一起监测大量样品,并且在手动计数过程中带有非常大的不确定性,特别是当构成的细胞克降巨细差异较大时,很难得到更有用,准确的数据.

三、比色法

TEC传感器 | 猫掌外链

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<section data-tools="135编辑器" data-id="95488" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; color: rgb(51, 51, 51); font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: 0.544px; orphans: 2; text-align: justify; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial;"><section style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; background-image: url("https://mmbiz.qpic.cn/mmbiz_gif/5rKWlTOSBibv7uvPes2ejZnfD6xxIXM4pqCZfoffSuYY1RYKbv5y9uM1fmrTN4oQNhse33Gh1TVV25gSKDXgWdA/640?wx_fmt=gif"); background-repeat: no-repeat; background-attachment: initial; background-origin: initial; background-clip: initial; background-position: center top; background-size: 100%;"><section style="margin: 0px; padding: 1em 2em 1em 0em; outline: 0px; max-width: 100%; box-sizing: border-box; word-wrap: break-word !important; font-size: 14px; letter-spacing: 1.5px; line-height: 1.75em; color: rgb(62, 62, 62);"><section data-autoskip="1" style="margin: 0px; padding: 0px 0px 1em 1em; outline: 0px; max-width: 100%; box-sizing: border-box; word-wrap: break-word !important; border-left: 1px solid rgb(44, 110, 213); border-bottom: 1px solid rgb(44, 110, 213);"><p style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; clear: both; min-height: 1em; vertical-align: inherit;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; color: rgb(136, 136, 136);">细胞的活性判定在生物学和医学上具有很重要的含义。它是体外细胞培养技能中常用的一个检测目标。不同的细胞活性判定办法有各自不同详细的反响机理，但不管选用哪种办法，都使用了死细胞和活细胞在生理机能和性质上的差异。常用的办法有直接染色法，克隆构成实验，比色法等。</span></p></section></section></section></section><section data-role="paragraph" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; color: rgb(51, 51, 51); font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: 0.544px; orphans: 2; text-align: justify; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial;"><p style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; clear: both; min-height: 1em; vertical-align: inherit;">下面咱们就简略的了解一下常用的细胞活性检测办法的原理，特色，以及各自的优缺陷。</p><section data-tools="135编辑器" data-id="108113" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"><section style="margin: 10px auto; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; text-align: center;"><section style="margin: 0px; padding: 0px 0px 25px 25px; outline: 0px; max-width: 100%; box-sizing: border-box; word-wrap: break-word !important; border-width: 1px; border-style: solid; border-color: rgb(102, 105, 169);"><section style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; display: flex; justify-content: flex-end;"><section style="margin: 0px; padding: 1px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; background-image: linear-gradient(to right top, rgb(102, 105, 169) 0%, rgb(102, 105, 169) 50%, rgb(255, 255, 255) 50%, rgb(255, 255, 255) 100%); background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; transform: translate(2px, -2px);"><section style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box; word-wrap: break-word !important; width: 15px; height: 15px; background-image: linear-gradient(to right top, rgb(191, 194, 233) 0%, rgb(191, 194, 233) 50%, rgb(255, 255, 255) 50%, rgb(255, 255, 255) 100%); background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; overflow: hidden;"><br style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"/></section></section></section><section data-brushtype="text" style="margin: 10px 0px 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; font-size: 14px; letter-spacing: 1.5px; color: rgb(102, 105, 169); text-align: left;">一、染色计数法</section><section data-width="70%" style="margin: 3px 0px 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box; word-wrap: break-word !important; width: 455px; height: 4px; background-color: rgb(102, 105, 169); overflow: hidden;"><br style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"/></section><section data-autoskip="1" data-role="list" style="margin: 15px 0px 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; text-align: justify; line-height: 1.75em; letter-spacing: 1.5px; font-size: 14px; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"><section data-role="list" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"><section data-role="list" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"><section data-role="list" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"><section data-role="list" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"><section data-role="list" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"><section data-role="list" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"><ol class=" list-paddingleft-2" style="margin: 0px; padding: 0px 0px 0px 30px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"><li><p style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; clear: both; min-height: 1em; vertical-align: inherit;">化学染色法<br style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"/></p><p style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; clear: both; min-height: 1em; vertical-align: inherit;">染色计数法是细胞培养中查看细胞死活zui常用的办法,直接使用死细胞和活细胞对染料的不同亲和力,查看细胞活性,能在光学显微镜下观察到染色成果.可分为两类:死细胞上色法和活细胞上色法.使死细胞上色的常用染料有台盼蓝,苯胺黑,伊红Y.能使活细胞上色的常用染料有结晶紫,亚甲基蓝,甲苯胺蓝等.其间zui常用的是台盼蓝染色法….细胞损害或逝世时,台盼蓝可穿透变性的细胞膜,与崩溃的DNA结合,使其上色,而活细胞能阻挠染料进入细胞内,故能够辨别死细胞与活细胞.经过细胞计数可得出细胞的存活率.</p><p style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; clear: both; min-height: 1em; vertical-align: inherit;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; caret-color:red color: rgb(192, 0, 0);">长处：</span><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; caret-color:red">便利有用，价格低廉，操作简略</span></p><p style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; clear: both; min-height: 1em; vertical-align: inherit;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; color: rgb(192, 0, 0);">缺陷：</span>时刻不宜过长,不然部分活细胞也会上色,会搅扰计数.并且,细胞没有经过固定,形状不明晰.</p><p style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; clear: both; min-height: 1em; vertical-align: inherit;"><br style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"/></p></li><li><p style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; clear: both; min-height: 1em; vertical-align: inherit;">荧光染色法</p><p style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; clear: both; min-height: 1em; vertical-align: inherit;">一些荧光染料对死细胞和活细胞也有不同的作用作用,使用荧光显微镜检测细胞活性.如碘化丙锭。<span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; caret-color:red">(PI),被活细胞排挤但能穿透正在逝世或现已逝世细胞的细胞膜,<span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; background-color: rgba(9, 187, 7, 0.31);">因</span>此活细胞不被染料染上色,只要死细胞或是凋亡细胞才干染上红.色.叶啶橙(AO)能透过质膜完好的细胞,嵌入细胞核DNA,使之宣布亮堂的绿色荧光.溴化乙锭(EB)仅能透过胞膜受损的细胞,嵌入核DNA,发橘红色荧光.也可使用AO—EB双染法判定细胞死活.</span></p><p style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; clear: both; min-height: 1em; vertical-align: inherit;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; caret-color:red"></span><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; caret-color:red color: rgb(192, 0, 0);">长处：</span><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; caret-color:red">这种检测办法比较传统的染料,具有灵敏度高,操作简洁,成果简单分辩等特色,并且使用双染法还能够分辩活细胞,凋亡前期细胞,凋亡晚期细胞,逝世细胞，在细胞凋亡的检测上有很广泛的使用。</span></p><p style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; clear: both; min-height: 1em; vertical-align: inherit;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; color: rgb(192, 0, 0);">缺陷：</span>要求特别的仪器进行检测,如荧光显微镜,激光扫描共聚集显微镜或流式细胞仪.并且一般荧光染料具有毒性,<span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; background-color: rgba(9, 187, 7, 0.31);">操作</span>作时需要带手套.<span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; caret-color:red"></span></p></li></ol></section></section></section></section></section></section></section></section></section></section><section data-tools="135编辑器" data-id="108113" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"><section style="margin: 10px auto; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; text-align: center;"><section style="margin: 0px; padding: 0px 0px 25px 25px; outline: 0px; max-width: 100%; box-sizing: border-box; word-wrap: break-word !important; border-width: 1px; border-style: solid; border-color: rgb(102, 105, 169);"><section style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; display: flex; justify-content: flex-end;"><section style="margin: 0px; padding: 1px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; background-image: linear-gradient(to right top, rgb(102, 105, 169) 0%, rgb(102, 105, 169) 50%, rgb(255, 255, 255) 50%, rgb(255, 255, 255) 100%); background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; transform: translate(2px, -2px);"><section style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box; word-wrap: break-word !important; width: 15px; height: 15px; background-image: linear-gradient(to right top, rgb(191, 194, 233) 0%, rgb(191, 194, 233) 50%, rgb(255, 255, 255) 50%, rgb(255, 255, 255) 100%); background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; overflow: hidden;"><br style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"/></section></section></section><section data-brushtype="text" style="margin: 10px 0px 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; font-size: 14px; letter-spacing: 1.5px; color: rgb(102, 105, 169); text-align: left;">二、克隆构成实验</section><section data-width="70%" style="margin: 3px 0px 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box; word-wrap: break-word !important; width: 455px; height: 4px; background-color: rgb(102, 105, 169); overflow: hidden;"><br style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"/></section><section data-autoskip="1" style="margin: 15px 0px 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; text-align: justify; line-height: 1.75em; letter-spacing: 1.5px; font-size: 14px; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"><p style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; clear: both; min-height: 1em; vertical-align: inherit;">克隆构成实验是测定单个细胞增值才能的有用办法之一,其基本原理是单个细胞在体外继续割裂增殖6 次以上,其子孙所组成的细胞集体,称为克隆或集落.一般状况,每个克降可含50个以上的细胞,巨细在0.3~~I.0mm之间.经过计数克隆构成率,可对单个细胞的增值潜力做定量分析.常见的办法有平板克隆构成实验,软琼脂构成实验等.这种办法常见于抗肿瘤药物敏感性实验,肿瘤放射生物学实验等.</p><p style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; clear: both; min-height: 1em; vertical-align: inherit;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; color: rgb(192, 0, 0);">长处：</span>在低剂量辐射下,克隆构成实验比 MTT和SRB法的敏感性更强.</p><p style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; clear: both; min-height: 1em; vertical-align: inherit;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; color: rgb(192, 0, 0);">缺陷：</span>较为繁琐并耗l时,不适合一起监测<span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; background-color: rgba(9, 187, 7, 0.31);">大</span>量样品,并且在手动计数过程中带有非常大的不确定性,特别是当构成的细胞克降巨细差异较大时,很难得到更有用,准确的数据.</p></section></section></section></section></section><section data-tools="135编辑器" data-id="108113" style="margin: 0px; padding: 0px; outline: currentcolor none 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; color: rgb(51, 51, 51); font-family: -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: 0.544px; text-align: justify; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial;"><section style="margin: 10px auto; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; text-align: center;"><section style="margin: 0px; padding: 0px 0px 25px 25px; outline: 0px; max-width: 100%; box-sizing: border-box; word-wrap: break-word !important; border-width: 1px; border-style: solid; border-color: rgb(102, 105, 169);"><section style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; display: flex; justify-content: flex-end;"><section style="margin: 0px; padding: 1px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; background-image: linear-gradient(to right top, rgb(102, 105, 169) 0%, rgb(102, 105, 169) 50%, rgb(255, 255, 255) 50%, rgb(255, 255, 255) 100%); background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; transform: translate(2px, -2px);"><section style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box; word-wrap: break-word !important; width: 15px; height: 15px; background-image: linear-gradient(to right top, rgb(191, 194, 233) 0%, rgb(191, 194, 233) 50%, rgb(255, 255, 255) 50%, rgb(255, 255, 255) 100%); background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; overflow: hidden;"><br style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"/></section></section></section><section data-brushtype="text" style="margin: 10px 0px 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important; font-size: 14px; letter-spacing: 1.5px; color: rgb(102, 105, 169); text-align: left;">三、比色法</section><section data-width="70%" style="margin: 3px 0px 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box; word-wrap: break-word !important; width: 455px; height: 4px; background-color: rgb(102, 105, 169); overflow: hidden;"><br style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; word-wrap: break-word !important;"/></section><section data-autoskip="1" style="margin: 15px 0px 0px; 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